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Mass Settings
Monoisotopic Mass

All calculated masses displayed in Mass Frontier are monoisotopic masses. The monoisotopic mass of an ion is the mass of the isotopic peak which is composed of the most abundant isotopes of its elements. Because the program’s calculations only support single-charged ions (z = 1), the calculated exact masses (not measured) in structure based modules are equal to their m/z value.

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Resolution

Resolution settings are used for the differentiation of adjacent peaks (spectra) and m/z values (calculations). Since Mass Frontier only processes centroid spectra, the differentiation is defined here as the spacing between resolved peaks (spectra) or as the smallest difference in m/z values that is distinguished (calculations). No additional parameters, such as peak width, are taken into account.

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Precision

Precision refers to m/z values and is defined here as the position of the last digit relative to the decimal point that is displayed. Precision settings are only used for the display of m/z values and peak positions on the m/z scale. In contrast to resolution, precision settings are ignored in all calculations as the highest possible precision is always used. To assure the correct display of m/z values, the precision must be set to the same or a higher order than the resolution.

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