The TECD (Total Extraction Component Detection) algorithm creates spectral trees for every section of a chromatogram that starts with an MS1 scan. Each generated tree is then divided into sub-trees based on the value of the Minimal Tree Depth parameter. Generated sub-trees are merged into components based on the precursor ion m/z value identity and the spectral tree match factor similarity.

To start automated component detection and the spectra deconvolution procedure using TECD algorithm:

1. Click on the Components Detection and Spectra Deconvolution button and choose the TECD pop-up menu item

2. When the parameters setup dialog window appears, change the settings if required, and then click the Calculate button

The TECD algorithm dialog window contains various parameters that can be optimized for specific types of analysis. Please note that these parameters are interdependent, so a change of one parameter can also affect algorithms linked with other parameters.

Minimal Tree Depth: Specifies the minimum number of tree sections the algorithm will create from the initial spectral tree. The value determines the MS stage where a division takes place.

Tree Match Factor: Specifies the minimum percentage that two spectral trees within adjacent tree sections must match before the algorithm will consider the two spectral trees as the same component. Matching spectral trees are defined as having identical precursors up to the level specified by the Minimal Tree Depth value and a Tree Match Factor value that exceeds the specified value.

Wide Component Merge Mode: Enables a comparison of the spectral trees for potential matching and merging, not only in adjacent sections, but also in sections up to the distance specified by the Allowed Gap value.

Allowed Gap: Specifies the maximum distance between nonadjacent tree sections over which to compare the spectral trees for potential merging.

Retention Time Range: Allows you to specify only a part of a chromatogram to be analyzed (time range), which can speed up your work. In this case, the algorithm ignores regions outside of the specified range

m/z Range: Allows you to specify only a part of a chromatogram to be analyzed (m/z range), which can speed up your work. In this case, the algorithm ignores regions outside of the specified range.